Fig 5. EcpH-ICAM blinking efficiency under different conditions.

HXB2 pseudoparticles co-labeled with Gag-imCherry and EcpH-ICAM were pre-bound to cells and imaged at 37°C for 60 min. Blinking events were detected as quenching/dequenching of EcpH-ICAM label. Plot shows the EcpH blinking efficiency measured in LCIB and HBSS in CV1/CD4/CXCR4 cells and in LCIB-bathed parental CV-1 cells lacking CD4. Total number of particles examined for each condition listed at top of plot. The fraction of particles exhibiting blinking behavior was normalized to the fraction of particles entering into acidic compartments, as evidenced by permanent EcpH-ICAM quenching (Fig 3D). *p<0.05, student’s t-test.