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. 2016 Feb 10;11(2):e0148944. doi: 10.1371/journal.pone.0148944

Fig 8. Characterization of spontaneous content release from labeled HXB2pp adhered to coverslips.

Fig 8

(A) Images of spontaneous content release from a single HXB2pp co-labeled with Gag-imCherry/EcpH-ICAM. Pseudoviruses were attached to poly-L-lysine coated glass coverslips in the cold and imaged in LCIB at 37°C. (B) Fluorescence intensity profiles of particle shown in panel A. (C) Comparison of content release efficiency from immobilized HXB2pp co-labeled with Gag-imCherry/EcpH-ICAM and Gag-imCherry/YFP-Vpr during 60 min image acquisition. (D) Kinetics of single content release events on poly-L-lysine coated glass and on CV1/CD4/CXCR4 cells shown as cumulative of events per total particles over time (symbols). (E) Distribution of immobilized HXB2pp on poly-L-lysine coated glass with background-subtracted EcpH intensity for all co-labeled particles (black) and co-labeled particles that spontaneously lyse (red). See also S3 Movie.