Figure 6.

Effect of methylation stress on pathogenic E. coli strain LF82 (AIEC). (A) Growth curves of AIEC strain LF82 in the presence (triangle) and in the absence (circles) of MMS. Superimposed to data points, for each growth curve, is the 4-parameter logistic regression following the formula y = y₀ + a/[1+(x/x₀)^b], where y₀ is the intercept with y-axis, a is the maximum value of y, x₀ is the mid-point, and b is the curvature coefficient. (B) Adhesion indexes of LF82 strain on Caco-2 cells after 4 h infection in the presence and in the absence of MMS. Values are reported as the mean adhesive bacteria (± standard error of the mean) per single Caco-2 cell. (C) Invasion indexes of LF82 strain on Caco-2 cells after 4 h infection in the presence and in the absence of MMS. Values are reported as the mean adhesive bacteria (± standard error of the mean) per single Caco-2 cell.