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ROS generation in promastigotes in the presence of amlodipine and fendiline. The ROS production was verified using the indicator 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA) after incubation with amlodipine and fendiline (20 μM). Nitazoxanide was used as positive control. (∗) indicates significant difference relative to the untreated group (∗ p < 0.05; ∗∗ p < 0.001; ∗∗∗ p < 0.001).
