Fig. 2.

The impacts of ectopic expression of CYB5D2 on serum-induced activation of AKT and ERK kinases. HeLa EV and HeLa CYB5D2 cells at approximately 90% confluency were serum starved for 24 h, and stimulated with 10% of fetal bovine serum for the indicated periods, followed by western blot analysis for the phosphorylation of AKT at serine 473 (pAKT) and ERK at threonine 183 and tyrosine 185 (pERK1/2) as well as CYB5D2 and GAPDH. Experiments were performed twice; typical results from a single repeat are shown. SF: serum free.