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. 2016 Jan 28;6:811–816. doi: 10.1016/j.dib.2016.01.036

Fig. 4.

Fig. 4.

Anti-CYB5D2 antibody specifically recognizes CYB5D2. Normal human kidney tissue was immunohistochemistry (IHC) stained with anti-CYB5D2 antibody without and with addition of recombinant GST-CYB5D2 or GST. The indicated regions were enlarged 3 fold and presented underneath of the individual panels. Recombinant GST-CYB5D2 and GST were produced in E. coli BL21. The recombinant protein GST-CYB5D2 was generated by N-terminal fusion of the transmembrane domain deletion mutant of CYB5D2 to GST. Anti-CYB5D2 antibody was affinity-purified by using GST-CYB5D2 as previously described [4]. For the competition experiments, GST-CYB5D2 or GST at 1 mg/ml was pre-incubated for one hour on ice with anti-CYB5D2 antibody (1:250) before applying to human kidney tissues.