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. 2016 Feb 11;6:20646. doi: 10.1038/srep20646

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Copyright © 2016, Macmillan Publishers Limited

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(A) A representative flow cytometric acquisition plot showing forward scatter (FSC) vs side scatter (SSC) of an NPC preparation, with the gate (NPC) indicated. Note the linear Y axis scale for SSC indicating smaller cells. (B) A two-color flow cytometric analysis of gated NPC for isotype controls FITC-rat IgG2b.κ and Alexa 594-goat IgG. (C) A two-color flow cytometric analysis of gated NPC using FITC-2.4G2 mab IgG anti-RIIb and Alexa 594-goat IgG anti-SR-B1. In (A–C) events are represented as blue points, with areas of very high density and high density colored red and yellow, respectively. The percentage of events showing single or double positive expression is indicated in respective quadrants. The results are representative of 4 different experiments.