Table 1. Comprehensive behavioral test battery of Rng105 +/− mice.
| Test | Age (weeks old) | Phenotypes in Rng105+/−mice | Results |
|---|---|---|---|
| Body weight, body temperature | 12 | NS | Sup. 2 |
| Wire hang | 12 | NS | Sup. 2 |
| Grip strength | 12 | NS | Sup. 2 |
| Light/dark transition | 12 | NS | Sup. 6 |
| Open field | 13 | NS | Sup. 3 |
| Elevated plus maze | 14 | NS | Sup. 6 |
| Hot plate | 14 | Reduced latency to withdraw their paws | Sup. 5 |
| Social interaction test in a novel environment | 14 | NS | Fig. 2 |
| Rotarod | 15 | NS | Sup. 4 |
| Three-chambered social approach | 17 | Reduced preference for social novelty | Fig. 2 |
| Startle response/prepulse inhibition | 18 | NS | Sup. 5 |
| Porsolt forced swim | 18 | Reduced immobility | Sup. 7 |
| Gait analysis | 19 | Reduced stride length | Sup. 4 |
| Barnes maze | 21–28 | Increased latency to reach the correct hole in reversal learning | Fig. 4 |
| Sup. 8 | |||
| T-maze (spontaneous alternation) | 33–34 | NS | Sup. 8 |
| Tail suspension | 35 | NS | Sup. 7 |
| Beam test | 38 | NS | Sup. 4 |
| Limb-clasping reflexes | 40 | Increased limb-clasping reflexes | Sup. 4 |
| Novel object recognition | 40 | Reduced preference for object novelty | Fig. 3 |
| T-maze (forced alternation) | 44–51 | Reduced latency and distance to finish the task | Fig. 4 |
| Sup. 8 | |||
| T-maze (left-right discrimination) | 51–53 | Reduced distance to finish the task in initial learning but not in reversal learning | Fig. 4 |
| Contextual and cued fear conditioning | 56–60 | NS | Sup. 8 |
| Social interaction test in a home cage | 60–61 | Reduced social interaction during active period | Fig. 2 |
| Marble burying test | 63 | NS | Sup. 6 |
| Place recognition test | 70 | Reduced motility in a novel chamber | Fig. 3 |
NS: no significant differences.