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. 2016 Feb 10;7:10619. doi: 10.1038/ncomms10619

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(a) Relationship between turnover rate enhancement factor after encapsulation against enzyme molecular weight (fitted using one-phase decay function). (b) Nanocage-encapsulated G6pDH activity change after incubation with different amount of NaCl. Assay conditions: 0.5 nM enzyme-DNA cage, incubation with 1 mM glucose-6-phosphate and 1 mM NAD⁺ in TBS buffer (pH 7.5), and monitoring absorbance at 340 nm. (c) Normalized k_cat and K_m values of free G6PDH and G6PDH that is encapsulated within different DNA cage: SH(G6pDH), a honeycomb lattice origami with a single layer; SS(G6pDH), a square-lattice origami with a single layer; and DS(G6pDH), a square-lattice origami with two layers. k_cat and K_m values of caged enzymes are normalized to that of free enzymes. Error bars were generated as the standard deviation of at least three replicates.