Table 1. Enzyme kinetic data for each individual enzyme encapsulated inside a DNA full-cage in comparison with the values for the free enzymes in solution.
| Enzyme | pI | Molecular weight | Substrate | Free enzymes |
Encapsulated enzymes |
||
|---|---|---|---|---|---|---|---|
| Km (μM) | kcat (s−1) | Km (μM) | kcat (s−1) | ||||
| GOx | 4.2 | 160 kDa | Glucose | 6,200±900 | 240±10 | 3,000±600 | 1,300±50 |
| HRP | 8.8 | 44 kDa | H2O2 | 2.3±0.5 | 32±1 | 4.3±0.6 | 290±5 |
| ABTS | 2,600±400 | 59±5 | 2,500±200 | 560±20 | |||
| G6pDH | 4.3 | 100 kDa | Glucose-6-phosphate | 220±20 | 130±3 | 310±30 | 460±10 |
| NAD+ | 510±50 | 100±3 | 590±40 | 480±10 | |||
| MDH | 10.0 | 70 kDa | NADH | 180±50 | 51±5 | 270±50 | 460±30 |
| LDH | 5.0 | 140 kDa | NADH | 7.2±1.3 | 46±2 | 17.0±1.5 | 190±5 |
| β-Gal | 4.1 | 465 kDa | RBG | 58.7±16.0 | 8.5±0.6* | 95.5±18.9 | 1.6±0.1* |
ABTS, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid); GOx, glucose oxidase; G6pDH, glucose-6-phosphate dehydrogenase; HRP, horseradish peroxidase; LDH, lactic dehydrogenase; MDH, malic dehydrogenase; pI, isoelectric point.
The Michaelis–Menten plots of each enzyme and the conditions of the enzyme activity measurements can be found in the Supplementary Figs. The pI values of the enzymes were obtained from brenda-enzymes.org (refs 65, 66, 67, 68).
*kcat values for β-Gal groups were not calibrated.