Figure 6. The location of NTF3 in Duroc and Meishan boars testes at different ages (immunohistochemical staining, 400×).
Sg-spermatogonia, PS-primary spermatocyte, SS-secondary spermatocyte, ST-spermatid. (a) Testis of 20-day-old Duroc boar. (b) Testis of 20-day-old Meishan boar. (c) Testis of 75-day-old Duroc boar. (d) Testis of 75-day-old Meishan boar. (e) Testis of 270-day-old Duroc boar. (f) Testis of 270-day-old Meishan boar. (g) Control group of Duroc boar. (f) Control group of Meishan boar. In (a–f), anti-NTF3 was used as the primary antibody, and in (g–h), normal goat serum were used as a negative control. The brown signal in the images represents the location of NTF3, and the blue parts represent the nucleus, stained by Hematoxylin Staining Solution. Bars = 50 μm.