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. 2016 Feb 11;6:20653. doi: 10.1038/srep20653

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Copyright © 2016, Macmillan Publishers Limited

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Alp7 potentiates tip tracking by Alp14 (A) Schematic of dual-TIRF assay. MTs are nucleated by immobilized Alexa488 labeled seeds using 6 μM S. pombe tubulin “doped” with 2.5% rhodamine labeled brain tubulin. (B) Alp14-GFP is introduced and a weak tip tracking signal is visible (white arrows). (C) Adding Alp7 enhances tip tracking by Alp14-GFP. (D) Adding Alp7 also significantly increases the mean plus end growth rate and decreases the catastrophe frequency, producing long-lived, fast growing MTs (Supplementary Movie 3) with no significant effect on minus ends (Table 2).

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