Table 1. The multi-Gaussian fitted peak position 
, calculated excluded volume 
, and calculated oligomer diameter 
in 24 h, 48 h, 72 h, and 96 h α-Syn only samples.
| Sample | 24 h |
48 h |
units | ||||
|---|---|---|---|---|---|---|---|
| cluster | OI | OII | OIII | OI | OII | OIII | – |
 |
32.1 ± 2.2 | – | – | 34.2 ± 5.1 | 46.9 ± 7.7 | 61.0 ± 4.7 | pA |
 |
45.9 ± 3.1 | – | – | 48.8 ± 7.3 | 67.0 ± 11.0 | 87.1 ± 6.7 | nm3 |
 |
4.4 ± 0.1 | – | – | 4.53 ± 0.2 | 5.0 ± 0.3 | 5.5 ± 0.1 | nm |
| Sample | 72 h | 96 h | units | ||||
| cluster | OII | OIII | OIV | OII | OIII | OIV | – |
 |
47.9 ± 2.4 | 60.7 ± 4.1 | 92.4 ± 20.3 | 43.2 ± 1.0 | 65.4 ± 4.7 | 98.3 ± 25.5 | pA |
 |
68.4 ± 3.4 | 86.7 ± 5.8 | 132.0 ± 29.0 | 61.7 ± 1.4 | 93.4 ± 6.7 | 140.4 ± 36.4 | nm3 |
 |
5.1 ± 0.1 | 5.5 ± 0.1 | 6.3 ± 0.5* | 4.9 ± 0.1 | 5.6 ± 0.1 | 6.4 ± 0.5* | nm |
The errors are fitting errors. The oligomers are regarded as spheres to calculate their diameter. (*the calculated diameter of OIV based on the sphere model may lead to an overestimation of the actual size, which are more likely have an asymmetric short rod-like structure due to the broad distribution of blockage of OIV56 and the TEM image in Supplementary Fig. 5d.)