Figure 4. Sensitivity to RabGDI extraction is not disturbed in prion-infected cells.

(a) Crude membranes of N2a and 22LN2a cells were incubated with 0 (in duplicate) or 3 μM (in triplicate) rab-GDI for 1 hour at 37 °C under constant shaking. Insoluble membrane fractions were separated from soluble fractions by centrifugation (100,000 × g; 10 min). Equal amounts of proteins from soluble and insoluble fractions were subjected to immunoblot analysis using anti-rab7, rab9 or rab11 antibodies. (b) Signals of soluble fractions were densitometrically evaluated and expressed as percentage of the total amount of the respective rab protein found in membrane preparations. Triplicate or duplicate values from one experiment were averaged. The averages of at least three independent experiments were statistically analysed using one-way ANOVA and post-hoc analysis with Tukey’s test (*p-value < 0.05; ns = not significant). Bars represent standard deviation.