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. 2016 Feb 11;6:21658. doi: 10.1038/srep21658

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N2a (upper panel), 22LN2a (middle panel) and 22LNa + Gli (lower panel) were incubated with lysotracker-red (red) for 30 min. Then cells were fixed and stained using an anti-lamp-1primary antibody followed by cy-2-conjugated secondary antibody (green). Yellow colour indicates co-localisation. Confocal images were taken using a Zeiss LSM710.