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. 2016 Feb 11;6:20882. doi: 10.1038/srep20882

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(A) HE and representative immunostaining images of IDH1, P53 and KI67 in diffuse astrocytomas. 200× (upper), 400× (bottom). (B) Representative IDH1 wild-type (WT), IDH1 (red arrow, R132H) and IDH1 (blue arrow, R132C) mutations were analyzed by Sanger sequencing. (C) Comparison of 5hmC level in DA cases between with IDH1 mutation and wild type by immunohistochemistry (IHC), DNA sequencing, or both IHC and DNA sequencing using the unpaired Student’s t-test. Error bar represents the standard error.