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. 2016 Feb 11;6:21502. doi: 10.1038/srep21502

Figure 9. Analysis of regulatory molecules at the protein level.

Figure 9

C57BL/6 mice were given intravitreal injections of PBS (control) or SA/Pam3. After 24 h, enucleated eyes were either embedded in OCT or fixed in paraffin. Cryosections were subjected to immunostaining (A), as described in material and methods. From a separate group, eyes were enucleated and lysates were made in PBS using a tissue lyser. Retinal lysates were subjected to Western blotting to validate protein expression (C, left panel). The band intensities were quantitated by densitometric analysis using Image J and presented as bar graph using β-actin as control (C right panel). Protein levels of inflammatory cytokines were assessed by ELISA (B). Paraffin embedded eyes were subjected to H&E staining for histological analysis (D). (*P < 0.05, **P < 0.005, ***P < 0.0005 ANOVA).