Table 1. Silencing of DCR2 gene suppressed accumulation of siRNAs derived from the RGDV genome.
Category | VCMs |
Leafhopper vector |
|||
---|---|---|---|---|---|
Control | dsGFP | dsDCR2 | dsGFP | dsDCR2 | |
Total small RNA readsa | 11835372 | 11057285 | 11509794 | 13769943 | 16327254 |
Unique small RNA readsa | 2446143 | 1576542 | 1856333 | 4607665 | 5049873 |
Total RGDV matched readsb (%c) | 307968 (2.6%) | 1375897 (12%) | 935630 (8%) | 134678 (1%) | 48623 (0.2%) |
Unique RGDV matched readsb (%c) | 42447 (1.7%) | 56906 (3.6%) | 48242 (2.1%) | 30225 (0.7%) | 16188 (0.3%) |
aNumber of small RNA sequences (18–32 nt).
bNumber of viral siRNAs perfectly matched to the RGDV genome.
cPercentage of viral siRNAs perfectly matched to the RGDV genome.