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. 2016 Feb 11;6:20896. doi: 10.1038/srep20896

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(A) Relative mRNA expression levels of E-cadherin, vimentin, twist and Snail in CD133 + CXCR4+ cancer cells by qPCR. (B) The mRNA expression levels of EMT related genes in mice liver cancer tissues were detected by qPCR. (C) Immunofluorescence analysis of E-cadherin and vimentin expression in CD133 + CXCR4+ cancer cells separated from HCT116 by FACS (Fluorescence microscopy ×400 magnification). (D) E-cadherin and vimentin expression in liver cancer tissue of mice were tested by immunohistochemistry under optical microscope ×200 magnification. (*P < 0.05; **P < 0.01).