Figure 6. Hdac3 cooperates with p300 to target differentially on the loci of OL and astrocyte-lineage specific genes.

A) RNA-seq transcriptome profiling changes between control and CKO-Olig1 optic nerves. Representative transcripts of OL (red) or astrocyte (blue)-enriched genes were shown.

B) The percentage of annotated astrocyte- and OL-enriched genes based on Transcriptome Database (Cahoy et al., 2008) among differentially expressed transcripts.

C) Heatmap of Hdac3-binding signals at OPCs and mOLs by ChIP-seq. The boxed areas using k-means clustering indicated unique Hdac3 binding loci in OPCs and mOL, respectively.

D) Temporal expression patterns of representative Hdac3-targeted genes in mOL were plotted against differentiation stages from an OL transcriptome database (Dugas et al., 2006).

E) A pie chart shows that Hdac3 targeting sites in OPCs are associated with both astrocyte- and OPC-enriched genes. The others represent Hdac3-targets that are not significantly enriched in OPCs or astrocytes based on the cell-type specific transcriptome profiles (Cahoy et al., 2008).

F,G) Heatmaps show expression patterns of representative OPC-enriched and astrocyte-enriched genes in the transcriptome of control and Hdac3 CKO-Olig1 optic nerves at P14 by RNA-seq.

H) ChIP-seq enrichment shows binding profiles of H3k27ac around Hdac3 peak summits in OPC and mOL.

I) ChIP-seq enrichment analysis shows binding profiles of Hdac3 and p300 around H3k27ac peak summits in OPCs.

J) The representative OPC-enriched gene loci that are targeted by Hdac3, P300 and H3k27ac marks.

K,L) The representative astrocyte-enriched gene loci that are targeted by Hdac3. See also Figure S6.