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. 2015 Nov 12;6(2):228–242. doi: 10.1016/j.stemcr.2015.10.007

Figure 6.

Figure 6

“NESC-TO-NTs” Conversion Models NTDs Caused by Folic Acid Deficiency

(A and B) Folate metabolism pathway. Blue names (A) indicate key enzymes involved in folate metabolism whose expression levels are higher in NESCs versus RGPCs or NESCs-DN (B).

(C) NT formation was closely correlated with FA concentrations.

(D) BrdU incorporation assays of NESCs in 2.5 μM FA (2.5FA), 4.5 μM FA (4.5FA), and the media without FA (W/O-NESCs).

(E) Down folds of stem cell markers when NESCs were cultured in the 2.5FA or 4.5FA relative to W/O-NESCs.

Data are showed as mean ± SD in (C), (D), and (E) (data from three independent experiments). ∗p < 0.05, and ∗∗p < 0.01 by Student’s t test between each. Data in (B) are from two independent experiments.