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. 2016 Feb 11;6:20989. doi: 10.1038/srep20989

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Representative merged images of confocal microphotographs of the fluorescence staining with the indicated primary antibodies and Texas-red phalloidin in PAECs expressing wild type (WT) or phosphorylation defective mutants (T18A + S19A, S19A, T18A) of MLC, before (Control) and 3 min after stimulation with 1 u ml⁻¹ thrombin (Thrombin). pMLC and ppMLC indicate the fluorescence images obtained with the anti-pMLC^S19 antibody and anti-pMLC^T18+S19 antibody, respectively. n = 5 (a,b), n = 2 (c).