Figure 3.

Classification of MSCs from Bone Marrow and Adipose Tissue

(A) Schematic overview of experimental design that led to the Epi-Tissue-Score.

(B) Scatterplot of mean DNAm levels in MSCs from bone marrow (BM) versus MSCs from adipose tissue (AT) in the training set (27K BeadChips; CpGs with more than 40% difference are indicated by red lines).

(C) Differential DNAm levels were plotted against the sum of variances within MSCs derived from either BM or AT.

(D) β Values (DNAm levels) of 12 CpGs that were selected by these criteria.

(E) Classification of the training dataset by the Epi-Tissue-Score. This score represents the difference of β values at cg27149093 (SLC41A2) and cg08124030 (TM4SF1).

(F) DNAm levels of the 12 selected CpGs in the validation dataset (450K BeadChips; in analogy to Figure 3D).

(G) Classification of the validation dataset by the Epi-Tissue-Score.

(H) Pyrosequencing analysis of DNAm at the two CpGs corresponding to the Epi-Tissue-Score in 22 MSC samples from BM and AT.

(I) Classification of pyrosequencing results by the Epi-Tissue-Score based on CpG in SLC41A2 and TM4SF1 as indicated.