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. 2016 Feb 9;6(2):213–227. doi: 10.1016/j.stemcr.2016.01.005

Figure 3.

Figure 3

CD1d-Restricted, α-GalCer-Specific Response and Cytokine Profile of Re-iNKT Cells

(A) IFN-γ and IL-4 production. Re-iNKT cells or parental iNKT cells were co-cultured with DCs in the presence of vehicle or 100 ng/ml α-GalCer.

(B) Re-iNKT cells were cultured with α-GalCer DCs in the presence of the indicated blocking Abs. Blockade was assessed based on IFN-γ production.

(C) α-GalCer-dependency and CD1d-restriction. Re-iNKT cells were cultured with C1R cells expressing mock or the indicated molecules in the presence of vehicle or 100 ng/ml α-GalCer.

(D) Cytokine profiles. Re-iNKT cells or parental iNKT cells were stimulated for 24 hr with plate-bound control immunoglobulin G (IgG) or anti-CD3 mAb (10 μg/ml), after which levels of the indicated cytokines in the culture supernatants were evaluated.

Data were run in triplicate, and the experiment was repeated three times. The results of one representative experiment are shown. Error bars depict mean ± SD.