Table 1.
Junction Sequences of TCR Genes in Reprogrammed and Redifferentiated Cells
| Clone | Productivity | TRAV | TRAJ | 3′ Va | P(N) | 5′ Ja | Frequency |
|---|---|---|---|---|---|---|---|
| V-J Junction Sequence of Rearranged TRA Genes in the Genome of CD4+iNKT-iPSC | |||||||
| 1 | productive | 10∗1 | 18∗01 | TGTGTGGTGAGCG | ACAGAGGCTCAACCCTGGGGAGGCTATACTTT | 5/8 productive | |
| productive | 30∗01 | 10∗01 | TG | TGGCACAGAGAGG | ACGGGAGGAGGAAACAAACTCACCTTT | 3/8 productive | |
| 2 | productive | 10∗01 | 18∗01 | TGTGTGGTGAGC | GACAGAGGCTCAACCCTGGGGAGGCTATACTTT | 1/1 productive | |
| 4 | productive | 10∗01 | 18∗01 | TGTGTGGTGAGC | GACAGAGGCTCAACCCTGGGGAGGCTATACTTT | 1/1 productive | |
| 8 |
productive | 10∗01 | 18∗01 | TGTGTGGTGAGCG | ACAGAGGCTCAACCCTGGGGAGGCTATACTTT | 5/10 productive | |
| productive | 30∗01 | 10∗01 | TG | TGGCACAGAGAGG | ACGGGAGGAGGAAACAAACTCACCTTT | 5/10 productive | |
| V-J Junction Sequence of Rearranged TRA mRNAs of Re-iNKT Cells | |||||||
| 1 | productive | 10∗1 | 18∗01 | TGTGTGGTGAGCG | ACAGAGGCTCAACCCTGGGGAGGCTATACTTT | 9/13 productive | |
| productive | 30∗01 | 10∗01 | TG | TGGCACAGAGAGG | ACGGGAGGAGGAAACAAACTCACCTTT | 4/13 productive | |
| 2 | productive | 10∗01 | 18∗01 | TGTGTGGTGAGC | GACAGAGGCTCAACCCTGGGGAGGCTATACTTT | 5/5 productive | |
| 4 | productive | 10∗01 | 18∗01 | TGTGTGGTGAGC | GACAGAGGCTCAACCCTGGGGAGGCTATACTTT | 2/2 productive | |
| 8 | productive | 10∗01 | 18∗01 | TGTGTGGTGAGCG | ACAGAGGCTCAACCCTGGGGAGGCTATACTTT | 6/12 productive | |
| productive | 30∗01 | 10∗01 | TG | TGGCACAGAGAGG | ACGGGAGGAGGAAACAAACTCACCTTT | 6/12 productive | |
| Clone | Productivity | TRBV | TRBD | TRBJ | 3′ Vb | N1-P-Db-N2 | 5′ Jb | Frequency |
|---|---|---|---|---|---|---|---|---|
| V-D-J Junction Sequence of Rearranged TRB Genes in Genome of CD4+iNKT-iPSC | ||||||||
| 1 | productive | 25-1∗01 | 1∗01 | 1-5∗01 | TGTGCCAGCAGTGAA | CTAGGGGAGAATTTG | CAGCCCCAGCATTTT | 6/6 productive |
| unproductive | – | 1∗01 | 1-6∗01 | – | GGGTGCCGATGGAAT | TTCACCCCTCCACTTT | ||
| 2 | productive | 25-1∗01 | 1∗01 | 2-3∗01 | TGTGCCAGCAGTG | GCAGGAGCCTAAA | CACAGATACGCAGTATTTT | 2/2 productive |
| unproductive | germline | 1∗01 | 2-7∗01 | TACAAAGCTGTAACATTGTG | GGGACAGGGGGCCGC | TCCTACGAGCAGTACTTCGGGCCGG | ||
| 4 | productive | 25-1∗01 | 1∗01 | 2-3∗01 | TGTGCCAGCAG | GGGATCCGGGACAGGGGCC | GATACGCAGTATTTT | 4/4 productive |
| unproductive | 11-1∗01 | – | 2-6∗01 | GGCAG | – | CAGG | ||
| 8 |
productive | 25-1∗01 | 1∗01 | 1-5∗01 | TGTGCCAGCAGTGAA | CTAGGGGAGAATTTG | CAGCCCCAGCATTTT | 6/6 productive |
| unproductive | – | 1∗01 | 1-6∗01 | – | GGGTGCCGATGGAAT | TTCACCCCTCCACTTT | ||
| V-D-J Junction Sequence of Rearranged TRB mRNAs of Re-iNKT Cells | ||||||||
| 1 | productive | 25-1∗01 | 1∗01 | 1-5∗01 | TGTGCCAGCAGTGAA | CTAGGGGAGAATTTG | CAGCCCCAGCATTTT | 5/5 productive |
| 2 | productive | 25-1∗01 | 1∗01 | 2-3∗01 | TGTGCCAGCAGTG | GCAGGAGCCTAAA | CACAGATACGCAGTATTTT | 5/6 productive |
| productive | 12-3∗01 | 2∗01 | 2-3∗01 | TGTGCCAGCAGTT | CTTCATCCACTCCCGTC | GATACGCAGTATTTT | 1/6 productive | |
| 4 | productive | 25-1∗01 | 1∗01 | 2-3∗01 | TGTGCCAGCAG | CGGATCCGGGACAGGGGCC | GATACGCAGTATTTT | 5/5 productive |
| unproductive | 7-9∗04 | 1∗01 | 2-1∗01 | AGAGA | CACAGGCAGGGAA | GGGCC | ||
| unproductive | 20-1∗03 | – | 2-2P∗01 | GATGG | – | AGAGG | ||
| 8 | productive | 25-1∗01 | 1∗01 | 1-5∗01 | TGTGCCAGCAGTGAA | CTAGGGGAGAATTTG | CAGCCCCAGCATTTT | 6/7 productive |
| productive | 20-1∗01 | 2∗01 | 2-7∗01 | TGCAGTGCTAGA | TCCTACTTGGGGGGAGAA | GAGCAGTACTTC | 1/7 productive | |