Table 1.
Experimental design.
| Group | Number of pregnant goats | Number of foetuses/kids | Days of gestation at the time of inoculation | Inoculum (IV) |
|---|---|---|---|---|
| G1 | 7 | 10 | 40 | Nc-Spain7 106 tachyzoites |
| G2 | 7 | 8 | 90 | Nc-Spain7 106 tachyzoites |
| G3 | 7 | 8 | 120 | Nc-Spain7 106 tachyzoites |
| G4 | 3–3–3 | 3–3–3 | 40–90–120a | PBS |
IV: intravenous route.
aIn G4, 3 goats correspond to each time-point of inoculation (40, 90 and 120) and were culled at the average day of abortion of each group (i.e., 10 (n = 2) and 20 (n = 1) dpi for G1; 31 dpi for G2, n = 2) or parturition of kids (55 dpi for G2, n = 1 and 16–20 dpi for G3, n = 3).