Table 2.
Identification of differentially expressed proteins under N deficiency in M. circinellodies WJ11 by MALDI-TOF/TOF analysis
| Spot | Accession | Protein name | Theoretical MW (Da)/pI | MASCOT score | No. matched | Coverage (%) | Fold change 24 h/6 h | Fold change 60 h/6 h |
|---|---|---|---|---|---|---|---|---|
| Nitrogen metabolism | ||||||||
| 1 | gi|511001426 | Glutamine synthetase | 39,973/5.61 | 73 | 2 (0) | 6 | 14.49 | 5.58 |
| 2 | gi|511003862 | Glutamine synthetase | 40,412/5.61 | 102 | 2 (2) | 11 | 15.04 | 2.79 |
| 3 | gi|511008284 | Saccharopepsin | 44,921/4.81 | 243 | 5 (2) | 17 | 37.86 | 60.50 |
| 4 | gi|511001898 | Saccharopepsin | 44,736/4.91 | 129 | 3 (0) | 8 | 36.84 | 91.88 |
| Amino acid metabolism | ||||||||
| 5 | gi|511002358 | Acetolactate synthase | 73,772/8.13 | 297 | 5 (1) | 10 | 0.02 | 1.03 |
| 6 | gi|511004294 | Dihydroxy-acid dehydratase | 64,148/6.27 | 432 | 8 (4) | 11 | 0.55 | 0.82 |
| 7 | gi|511002359 | Ketol-acid reductoisomerase | 44,298/7.72 | 562 | 9 (3) | 27 | 0.10 | 0.17 |
| 8 | gi|511004962 | Branched-chain amino acid aminotransferase | 43,753/6.62 | 474 | 6 (3) | 19 | 0.38 | 0.59 |
| 9 | gi|511010054 | Acetylornithine aminotransferase | 49,342/8.32 | 123 | 4 (1) | 15 | 0.75 | 0.37 |
| 10 | gi|511006376 | Asparagine synthetase | 64,754/5.81 | 88 | 3 (0) | 8 | 0.15 | 0.80 |
| 11 | gi|511005296 | Saccharopine dehydrogenase | 41,899/5.36 | 471 | 12 (3) | 35 | 0.38 | 0.29 |
| 12 | gi|511002126 | Homoisocitrate dehydrogenase | 41,047/6.28 | 102 | 3 (0) | 10 | 0.29 | 0.15 |
| 13 | gi|511009910 | Serine hydroxymethyltransferase 2 | 51,589/6.53 | 193 | 8 (2) | 24 | 0.39 | 0.31 |
| 14 | gi|511001743 | Serine hydroxymethyltransferase, mitochondrial | 55,707/8.97 | 109 | 4 (1) | 11 | 0.30 | 0.22 |
| 15 | gi|511000252 | Glycine cleavage system T protein | 43,694/8.77 | 82 | 4 (0) | 11 | 0.18 | 0.47 |
| 16 | gi|511004260 | 3-Deoxy-7-phosphoheptulonate synthase | 38,738/6.46 | 239 | 4 (1) | 9 | 0.20 | 0.10 |
| 17 | gi|511010625 | S-Adenosylmethionine synthase | 42,413/5.67 | 70 | 1 (1) | 3 | 5.39 | 3.55 |
| 18 | gi|511010625 | S-Adenosylmethionine synthase | 42,413/5.67 | 106 | 1 (1) | 3 | 3.22 | 1.41 |
| Carbon metabolism | ||||||||
| 19 | gi|511002640 | Fructose-bisphosphate aldolase | 39,791/5.36 | 145 | 2 (2) | 11 | 1.64 | 2.99 |
| 20 | gi|511002640 | Fructose-bisphosphate aldolase | 39,791/5.36 | 236 | 3 (1) | 11 | 1.55 | 1.51 |
| 21 | gi|511008895 | Glyceraldehyde-3-phosphate dehydrogenase 1 | 35,588/6.09 | 88 | 3 (0) | 10 | 2.77 | 7.56 |
| 22 | gi|511008895 | Glyceraldehyde-3-phosphate dehydrogenase 1 | 35,588/6.09 | 106 | 2 (0) | 5 | 8.24 | 13.63 |
| 23 | gi|511008895 | Glyceraldehyde-3-phosphate dehydrogenase 1 | 35,588/6.09 | 394 | 5 (3) | 15 | 2.50 | 3.50 |
| 24 | gi|511007306 | Glyceraldehyde-3-phosphate dehydrogenase 2 | 36,013/5.54 | 550 | 10 (5) | 35 | 2.91 | 1.69 |
| 25 | gi|511004614 | Enolase | 47,164/5.56 | 385 | 5 (2) | 10 | 1.53 | 7.99 |
| 26 | gi|511006733 | Pyruvate kinase | 59,079/6.19 | 464 | 8 (3) | 18 | 7.59 | 6.44 |
| 27 | gi|511006733 | Pyruvate kinase | 59,079/6.19 | 109 | 4 (0) | 3 | 4.23 | 3.97 |
| 28 | gi|511004430 | Pyruvate dehydrogenase complex | 52,323/6.11 | 170 | 2 (1) | 5 | 17.82 | 1.36 |
| 29 | gi|511004430 | Pyruvate dehydrogenase complex | 53,161/5.76 | 123 | 2 (1) | 5 | 21.82 | 4.58 |
| 30 | gi|511005313 | Pyruvate dehydrogenase E1 component subunit alpha | 43,770/8.31 | 246 | 6 (2) | 11 | 6.89 | 1.35 |
| 31 | gi|511002644 | Pyruvate dehydrogenase E2 component | 53,161/5.76 | 183 | 4 (1) | 8 | 1.93 | 1.51 |
| 32 | gi|511002644 | Pyruvate dehydrogenase E2 component | 53,161/5.76 | 288 | 4 (2) | 8 | 50.48 | 21.50 |
| 33 | gi|511003312 | 2,3-Bisphosphoglycerate-independent phosphoglycerate mutase | 56,737/5.23 | 332 | 8 (3) | 16 | 0.48 | 0.41 |
| 34 | gi|511003312 | 2,3-Bisphosphoglycerate-independent phosphoglycerate mutase | 56,737/5.23 | 118 | 3 (0) | 5 | 0.46 | 0.62 |
| 35 | gi|511010411 | Glucose-6-phosphate dehydrogenase | 58,358/6.72 | 96 | 3 (0) | 12 | 1.62 | 1.51 |
| 36 | gi|511005381 | Transaldolase | 35,798/5.45 | 137 | 3 (0) | 9 | 2.64 | 0.71 |
| 37 | gi|511001250 | Transketolase | 74,273/5.71 | 108 | 4 (1) | 8 | 2.64 | 2.56 |
| 38 | gi|511005018 | NAD+:isocitrate dehydrogenase, mitochondrial | 41,006/7.02 | 735 | 11 (7) | 34 | 0.53 | 0.62 |
| 39 | gi|511005320 | NADP+:isocitrate dehydrogenase, mitochondrial | 47,644/5.97 | 164 | 4 (1) | 11 | 0.31 | 0.57 |
| 40 | gi|511002271 | Succinyl-CoA ligase, mitochondrial | 47,555/5.89 | 138 | 4 (1) | 12 | 0.55 | 0.39 |
| 41 | gi|511009244 | Succinate dehydrogenase | 71,166/6.15 | 112 | 3 (1) | 7 | 0.71 | 0.55 |
| 42 | gi|511006629 | Fumarate hydratase, mitochondrial | 53,753/6.65 | 410 | 4 (4) | 11 | 0.55 | 0.39 |
| 43 | gi|511003684 | Aldehyde dehydrogenase | 54,639/5.51 | 368 | 10 (4) | 20 | 1.52 | 1.95 |
| 44 | gi|511001067 | Aldehyde dehydrogenase | 53,854/5.57 | 284 | 5 (2) | 14 | 1.64 | 1.72 |
| 45 | gi|511008662 | Acetyl-CoA C-acetyltransferase | 41,434/5.65 | 507 | 8 (4) | 23 | 0.39 | 0.43 |
| 46 | gi|511005081 | Phosphoglucomutase | 61,303/5.61 | 151 | 4 (1) | 11 | 3.50 | 4.55 |
| 47 | gi|511006219 | UTP-glucose-1-phosphate uridylyltransferase | 56,716/6.15 | 655 | 10 (3) | 23 | 3.21 | 4.01 |
| 48 | gi|511006325 | UDP-glucose 4-epimerase | 39,235/5.76 | 138 | 3 (0) | 12 | 1.07 | 1.55 |
| 49 | gi|511009038 | Galactokinase | 48,847/6.00 | 209 | 5 (0) | 12 | 2.17 | 2.26 |
| 50 | gi|511010174 | S-Formylglutathione hydrolase | 32,160/6.11 | 61 | 2 (0) | 3 | 2.27 | 2.68 |
| 51 | gi|758354396 | Thiazole biosynthetic enzyme | 33,882/5.28 | 345 | 3 (1) | 18 | 20.96 | 14.71 |
| Protein metabolism | ||||||||
| 52 | gi|511000792 | 40S ribosomal protein S21 | 9718/4.93 | 287 | 3 (2) | 66 | 0.34 | 0.35 |
| 53 | gi|511011908 | 40S ribosomal protein S7 | 21,057/9.70 | 71 | 1 (1) | 12 | 0.07 | 0.12 |
| 54 | gi|511008091 | Eukaryotic translation initiation factor 5A | 17,694/5.08 | 302 | 3 (3) | 19 | 0.13 | 0.68 |
| 55 | gi|511011759 | Elongation factor Tu | 51,841/6.26 | 294 | 5 (2) | 13 | 0.17 | 0.09 |
| 56 | gi|511006438 | Nascent polypeptide-associated complex subunit beta | 17,501/5.34 | 362 | 5 (3) | 48 | 0.02 | 0.06 |
| 57 | gi|511003551 | 26S protease regulatory subunit 6A | 48,455/4.96 | 346 | 9 (2) | 18 | 0.29 | 0.33 |
| 58 | gi|511009637 | 26S protease regulatory subunit 6B | 46,545/5.72 | 228 | 4 (2) | 15 | 0.42 | 0.38 |
| 59 | gi|511010098 | 20S proteasome subunit Alpha 6 | 31,144/5.06 | 254 | 4 (2) | 15 | 0.01 | 0.02 |
| Nucleic acid metabolism | ||||||||
| 60 | gi|511005974 | Phosphoribosylaminoimidazole-succinocarboxamide synthase | 33,792/5.10 | 231 | 3 (2) | 11 | 0.49 | 0.60 |
| 61 | gi|511011778 | Adenylyl-sulfate kinase | 22,883/5.75 | 387 | 8 (2) | 32 | 0.20 | 0.12 |
| 62 | gi|511006145 | Dihydroorotase, homodimeric type | 39,652/5.83 | 233 | 5 (0) | 13 | 0.43 | 0.45 |
| Energy metabolism | ||||||||
| 63 | gi|511006698 | Adenylate kinase 1 | 28,268/7.01 | 624 | 10 (6) | 51 | 1.61 | 2.53 |
| 64 | gi|511006698 | Adenylate kinase 1 | 28,268/7.01 | 571 | 8 (5) | 29 | 37.78 | 20.29 |
| 65 | gi|511003961 | V-type H+-transporting ATPase subunit I | 95,461/5.05 | 193 | 3 (1) | 5 | 1.61 | 1.51 |
| 66 | gi|511007631 | V-type proton ATPase catalytic subunit A | 70,756/5.07 | 361 | 6 (3) | 15 | 3.23 | 1.72 |
| Signal transduction | ||||||||
| 67 | gi|511007036 | 14-3-3 family protein epsilon | 29,228/4.84 | 175 | 5 (1) | 24 | 1.75 | 2.01 |
| Molecular chaperone | ||||||||
| 68 | gi|511004981 | Heat shock 70 kDa protein 1/8 | 67,760/5.10 | 280 | 3 (2) | 7 | 4.23 | 3.64 |
| 69 | gi|511004335 | Hsp70-like protein | 71,389/5.79 | 202 | 4 (1) | 7 | 2.38 | 2.99 |
| 70 | gi|511003138 | Peptidyl-prolyl cis–trans isomerase cyp5 | 19,078/6.92 | 477 | 8 (5) | 47 | 1.72 | 1.64 |
| Redox homeostasis | ||||||||
| 71 | gi|511008071 | Peroxiredoxin | 22,926/4.94 | 254 | 3 (2) | 25 | 20.46 | 2.95 |
| 72 | gi|511008071 | Peroxiredoxin | 22,926/4.94 | 114 | 2 (1) | 12 | 15.30 | 13.16 |
| 73 | gi|511008071 | Peroxiredoxin | 22,926/4.94 | 173 | 3 (1) | 14 | 16.70 | 17.01 |
| 74 | gi|503389911 | Glutathione peroxidase | 20,881/6.40 | 61 | 1 (0) | 5 | 1.58 | 3.42 |
| 75 | gi|511008344 | Oxidoreductase | 30,743/6.25 | 432 | 5 (2) | 15 | 1.55 | 3.44 |
| 76 | gi|511009098 | Oxidoreductase | 28,164/6.54 | 310 | 6 (2) | 28 | 1.84 | 1.66 |
| 77 | gi|511009792 | Ferritin heavy chain | 20,301/5.23 | 268 | 5 (3) | 39 | 2.30 | 2.08 |
| Hypothetical protein | ||||||||
| 78 | gi|511011920 | Hypothetical protein HMPREF1544_00199 | 91,261/6.42 | 211 | 5 (1) | 5 | 23.83 | 8.50 |
| 79 | gi|511010665 | Hypothetical protein HMPREF1544_01177 | 55,006/4.72 | 85 | 2 (1) | 6 | 4.84 | 2.65 |
| 80 | gi|511010454 | Hypothetical protein HMPREF1544_01386 | 28,881/6.42 | 101 | 2 (0) | 6 | 41.34 | 58.10 |
| 81 | gi|511009952 | Hypothetical protein HMPREF1544_01888 | 14,839/5.89 | 125 | 1 (1) | 10 | 4.23 | 2.59 |
| 82 | gi|511009952 | Hypothetical protein HMPREF1544_01888 | 14,839/5.89 | 293 | 3 (2) | 32 | 3.18 | 1.74 |
| 83 | gi|511009799 | Hypothetical protein HMPREF1544_02101 | 47,569/7.79 | 191 | 3 (2) | 10 | 0.35 | 0.43 |
| 84 | gi|511009692 | Hypothetical protein HMPREF1544_02181 | 22,351/5.33 | 175 | 3 (2) | 13 | 23.25 | 37.32 |
| 85 | gi|511009605 | Hypothetical protein HMPREF1544_02260 | 26,248/5.37 | 175 | 3 (1) | 12 | 9.70 | 3.04 |
| 86 | gi|511008946 | Hypothetical protein HMPREF1544_03035 | 42,241/7.22 | 630 | 8 (4) | 26 | 34.49 | 15.14 |
| 87 | gi|511008765 | Hypothetical protein HMPREF1544_03136 | 53,353/5.77 | 422 | 5 (4) | 15 | 0.06 | 0.07 |
| 88 | gi|511008618 | Hypothetical protein HMPREF1544_03262 | 68,758/6.29 | 378 | 7 (3) | 12 | 0.01 | 0.01 |
| 89 | gi|511008350 | Hypothetical protein HMPREF1544_03549 | 43,856/7.82 | 229 | 4 (2) | 9 | 13.89 | 17.99 |
| 90 | gi|511008286 | Hypothetical protein HMPREF1544_03639 | 48,153/5.50 | 235 | 5 (2) | 10 | 0.11 | 0.25 |
| 91 | gi|511008150 | Hypothetical protein HMPREF1544_03790 | 20,026/5.04 | 307 | 4 (2) | 22 | 0.16 | 0.34 |
| 92 | gi|511008020 | Hypothetical protein HMPREF1544_03919 | 29,429/6.97 | 425 | 6 (4) | 37 | 0.20 | 0.19 |
| 93 | gi|511007940 | Hypothetical protein HMPREF1544_03956 | 29,843/5.14 | 123 | 2 (1) | 10 | 25.34 | 20.07 |
| 94 | gi|511007175 | Hypothetical protein HMPREF1544_04707 | 63,905/5.35 | 106 | 2 (0) | 4 | 0.09 | 0.09 |
| 95 | gi|511006887 | Hypothetical protein HMPREF1544_05024 | 55,228/6.28 | 275 | 3 (2) | 9 | 2.16 | 5.78 |
| 96 | gi|511006887 | Hypothetical protein HMPREF1544_05024 | 55,228/6.28 | 309 | 5 (2) | 10 | 5.47 | 5.35 |
| 97 | gi|511006017 | Hypothetical protein HMPREF1544_05863 | 30,010/5.58 | 423 | 4 (4) | 23 | 2.45 | 2.18 |
| 98 | gi|511004978 | Hypothetical protein HMPREF1544_06871 | 18,967/4.96 | 249 | 3 (2) | 13 | 5.27 | 17.90 |
| 99 | gi|511004902 | Hypothetical protein HMPREF1544_06986 | 28,947/9.05 | 103 | 1 (1) | 6 | 3.61 | 2.41 |
| 100 | gi|511004522 | Hypothetical protein HMPREF1544_07306 | 26,114/5.56 | 252 | 4 (2) | 21 | 7.99 | 3.58 |
| 101 | gi|511004398 | Hypothetical protein HMPREF1544_07438 | 21,313/5.70 | 199 | 3 (1) | 13 | 0.29 | 0.18 |
| 102 | gi|511004099 | Hypothetical protein HMPREF1544_07752 | 21,249/5.14 | 294 | 6 (2) | 31 | 24.54 | 3.86 |
| 103 | gi|511003967 | Hypothetical protein HMPREF1544_07893 | 16,356/4.59 | 152 | 3 (1) | 30 | 0.04 | 0.20 |
| 104 | gi|511003188 | Hypothetical protein HMPREF1544_08616 | 30,249/5.39 | 224 | 4 (1) | 11 | 28.24 | 26.23 |
| 105 | gi|511003039 | Hypothetical protein HMPREF1544_08758 | 19,098/8.39 | 392 | 4 (3) | 25 | 4.40 | 2.25 |
| 106 | gi|511003039 | Hypothetical protein HMPREF1544_08758 | 19,098/8.39 | 347 | 8 (2) | 70 | 8.61 | 9.91 |
| 107 | gi|511002615 | Hypothetical protein HMPREF1544_09175 | 26,203/5.36 | 170 | 4 (1) | 22 | 6.71 | 3.31 |
| 108 | gi|511002606 | Hypothetical protein HMPREF1544_09235 | 58,903/4.89 | 98 | 1 (1) | 5 | 23.78 | 30.46 |
| 109 | gi|511002606 | Hypothetical protein HMPREF1544_09235 | 58,903/4.89 | 81 | 2 (0) | 5 | 5.99 | 20.72 |
| 110 | gi|511001781 | Hypothetical protein HMPREF1544_10038 | 43,811/6.02 | 96 | 1 (1) | 4 | 8.25 | 14.78 |
| 111 | gi|511001506 | Hypothetical protein HMPREF1544_10287 | 14,404/4.95 | 156 | 3 (0) | 26 | 24.12 | 22.30 |
| 112 | gi|511001144 | Hypothetical protein HMPREF1544_10622 | 24,389/5.25 | 162 | 3 (1) | 15 | 5.08 | 4.08 |
| 113 | gi|511001065 | Hypothetical protein HMPREF1544_10686 | 38,562/6.58 | 161 | 5 (0) | 20 | 26.39 | 17.31 |
| 114 | gi|511000884 | Hypothetical protein HMPREF1544_10852 | 100,320/8.41 | 161 | 3 (1) | 5 | 0.04 | 0.00 |
| 115 | gi|511000884 | Hypothetical protein HMPREF1544_10852 | 100,320/8.41 | 484 | 6 (4) | 10 | 0.04 | 0.01 |
| 116 | gi|511000884 | Hypothetical protein HMPREF1544_10852 | 10,0320/8.41 | 210 | 4 (1) | 7 | 0.01 | 0.02 |
| 117 | gi|511000397 | Hypothetical protein HMPREF1544_11327 | 66,634/5.41 | 127 | 3 (0) | 8 | 24.47 | 16.26 |
| 118 | gi|511000331 | Hypothetical protein HMPREF1544_11393 | 14,081/6.75 | 138 | 3 (1) | 38 | 2.61 | 3.05 |