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. 2016 Feb 11;16:77. doi: 10.1186/s12879-016-1410-9

Table 3.

Virucidal activity of the “final formulation” with 2 % urea and 2 % citric acid against test viruses of the German (values with Aqua bidest. and FCS) and European Guidelines (values with clean conditions)

Virus Conc. Test method Soil load Exposure times
15 s 30 s 60 s 90 s
BVDV 80 % DVV/RKI Aqua bidest. ≥4.63 ± 0.16 ≥4.63 ± 0.16 n.d. n.d.
BVDV 80 % DVV/RKI FCS ≥4.63 ± 0.16 ≥4.63 ± 0.16 n.d. n.d.
Vaccinia virus 80 % DVV/RKI Aqua bidest. ≥5.44 ± 0.19 ≥5.44 ± 0.19 ≥5.44 ± 0.19 n.d.
Vaccinia virus 80 % DVV/RKI FCS ≥4.94 ± 0.26 ≥5.51 ± 0.18 ≥5.51 ± 0.18 n.d.
PV 80 % DVV/RKI Aqua bidest. n.d. 4.32 ± 0.41 ≥6.13 ± 0.35 n.d.
PV 80 % DVV/RKI FCS n.d. 3.13 ± 0.37 4.57 ± 0.37 n.d.
PV 80 % EN 14476 Clean conditions n.d. ≥5.32 ± 0.34 ≥5.75 ± 0.30 n.d.
AdV 80 % DVV/RKI Aqua bidest. n.d. 3.75 ± 0.31 ≥4.50 ± 0.29 ≥5.13 ± 0.33
AdV 80 % DVV/RKI FCS n.d. 3.44 ± 0.00 4.31 ± 0.42 4.94 ± 0.40
AdV 80 % EN 14476 Clean conditions n.d. 4.19 ± 0.31 ≥5.38 ± 0.25 n.d.
SV40 80 % DVV/RKI Aqua bidest. n.d. ≥5.44 ± 0.27 ≥5.44 ± 0.27 ≥5.88 ± 0.29
SV40 80 % DVV/RKI FCS n.d. 3.75 ± 0.55 4.32 ± 0.34 ≥5.38 ± 0.25
MNV 80 % EN 14476 Clean conditions n.d. 4.13 ± 0.39 ≥5.38 ± 0.29 ≥5.88 ± 0.33

Results are derived from a quantitative suspension test in duplicates and presented as reduction factor (RF) with 95 % confidence interval. n.d. not determined