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. 2016 Feb 11;5:7. doi: 10.1186/s13630-016-0028-2

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© Dummer et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — The PyT and the DAAS methods. a. PyT method. With the assumption of a continuous slope, the Pythagorean theorem can be applied to calculate the length of a cilium. b–d. DAAS method. After selecting a cilium and the cross section line (b), the program gives a top view (c) and the vertical slice of the selected plane (d). Measurements are performed in Matlab. Cilia are measured on grayscales from 55 to 255