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. 2016 Jan 25;6:19005. doi: 10.1038/srep19005

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(A) Nucleotide sequence analysis. The indicated promoter region, 409 bp upstream and 104 bp downstream of the start codon (double-underlined), was fused with lacZ. Transcriptional start site (TSS, indicated by asterisks) is located 120 bp upstream from the start codon of the bclA gene. The SigK consensus sequence is indicated with a gray box, and the putative -35 and -10 sequences are underlined. (B) β-galactosidase activity assay of PbclA in wild-type HD73 (▲), sigK mutant (◼), and gerE mutant (●). T₀ is the end of the exponential phase, and T_n is n hours after T₀. Values represent mean of at least three independent replicates; error bars represent standard deviation.