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. Author manuscript; available in PMC: 2016 Feb 11.

Published in final edited form as: Ann Otol Rhinol Laryngol. 2015 Jun 15;124(11):893–902. doi: 10.1177/0003489415590657

Wound healing, or cell migration, was measured in confluent cultures of esophageal epithelial cells cultured from human biopsy via scratch assay. All pepsin alone (no acid) treatment conditions were initiated post-scratch and extended throughout the migration assay observation period; all pH4 treatments were limited to five minutes immediately prior to scratch because of the highly damaging nature of acid treatment. Cell migration into the area of the wound was monitored using an Essen Bioscience Live Cell Imaging System which provided (A) masks of the border of the initial wound (black) and cell migration progress (gray; 6 hours post-scratch shown, scale bar=300um), and (B) Relative Wound Density (RWD) as a metric of cell migration.

Wound healing, or cell migration, was measured in confluent cultures of esophageal epithelial cells cultured from human biopsy via scratch assay. All pepsin alone (no acid) treatment conditions were initiated post-scratch and extended throughout the migration assay observation period; all pH4 treatments were limited to five minutes immediately prior to scratch because of the highly damaging nature of acid treatment. Cell migration into the area of the wound was monitored using an Essen Bioscience Live Cell Imaging System which provided (A) masks of the border of the initial wound (black) and cell migration progress (gray; 6 hours post-scratch shown, scale bar=300um), and (B) Relative Wound Density (RWD) as a metric of cell migration.