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. Author manuscript; available in PMC: 2016 Feb 11.
Published in final edited form as: Ann Otol Rhinol Laryngol. 2015 Jun 15;124(11):893–902. doi: 10.1177/0003489415590657

Figure 5. Migration assay 30 minutes and 24 hours following 5 minute acid or acid and pepsin exposure.

Figure 5

Esophageal cells (A) 30 minutes and (B) 24 hours after assay start. Cells were treated with pepsin alone throughout the duration of the assay; acid (pH4) treatment was limited to 5 minutes pretreatment prior to assay start at which time cells were rinsed and normal growth media was replaced. Bubbles observed in (A) are a normal product of media transfer that disappeared by subsequent image collection 2hours post-treatment.