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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Nutr Biochem. 2015 Sep 21;27:211–218. doi: 10.1016/j.jnutbio.2015.09.008

Figure 1.

Figure 1

Schematic representing the sampling strategy utilized in this study. The first layer of stratification separated untreated conceptuses from those treated with MEHP. Visceral yolk sacs were removed from each intact concept and evaluated separately at each stratification level. Control embryos exhibiting open neural tubes (NTD) occur normally at a very low incidence. Only control conceptuses without NTD [Control (−)] were used in this stratification model. MEHP-treated conceptuses were further separated according to their NTD status. Those with embryos exhibiting an open neural tube [MEHP (+)] were separated from exposed conceptuses where no open neural tube was observed [MEHP (−)].