Table 1.
Measurements of dietary methyl donors and C1 metabolism components in whole conceptuses following 24 h in WEC. All measurements were normalized, and are reported as the concentration of each nutrient per mg total protein.
| Control | MEHP (100 µg/ml) | MEHP (250 µg/ml) | |
|---|---|---|---|
| Folate (ng/mg protein) | 0.66±0.04 (n=4) |
0.51±0.03 (n=4) |
0.74±0.08 (n=5) |
| Vitamin B12 (pg/mg protein) | 17.00±0.09 (n=3) |
12.25±0.41*** (n=3) |
15.23±0.47*$$ (n=5) |
| Choline (ng/mg protein) | 2.15±0.41 (n=5) |
1.35±0.54 (n=5) |
3.04±0.87$$ (n=4) |
| Betaine (ng/mg protein) | 4.23±0.39 (n=4) |
2.13±0.95** (n=5) |
2.95±0.42 (n=4) |
| SAM (ng/mg protein) | 820.93±87.27 (n=4) |
1487.44±358.57 (n=5) |
2380.10±659.50 (n=5) |
| Cysteine (ng/mg protein) | 1467.39±192.00 (n=5) |
2338.37±377.96 (n=5) |
2506.89±265.18 (n=5) |
| Homocysteine (ng/mg protein) | 70.10±5.07 (n=5) |
63.75±3.36 (n=4) |
97.37±8.50*$$ (n=4) |
| Methionine (ng/mg protein) | 635.06±43.95 (n=4) |
956.51±171.79 (n=5) |
1426.98±235.62* (n=5) |
| Total protein (mg/sample) | 2.68±0.09 (n=5) |
2.84±0.09 (n=5) |
2.40±0.11$ (n=5) |
Comparisons between treatments and controls:
*
p<0.05
**
p<0.01
***
p<0.001.
Comparisons between low- and high-dose groups:
$
p<0.05
$$
p<0.01.