Figure 4.

Altered cytokine response in the absence of IL-17A during VL. Cytokine response was evaluated in splenocyte supernatants of WT or IL-17A^−/− mice after 15 or 30 dpi. Splenocytes were plated at 5 × 10⁵ cells/well in 96 well plates and were stimulated or not with L. donovani soluble antigen (20 µg/ml). After 72 h the cell supernatants were recovered and analyzed by ELISA for IL-4 (A), IL-6 (B), IL-10 (C), or IL-13 (D) production. Data represent 1 of 2 independent experiments with similar results. **P < 0.01.