Figure 5.

Enhanced IFN-γ production in IL-17A-deficient mice. IFN-γ response was evaluated in splenocytes of infected WT or IL-17A^−/−-deficient mice. A) IFN-γ detection by ELISA after 72 h of restimulation with L. donovani soluble antigen (20 µg/ml) at 15 or 30 dpi. B) Intracellular detection of IFN-γ by flow cytometry at 15 dpi. Splenocytes were stimulated with L. donovani soluble antigen (20 µg/ml) for 18 h and with ionomycin/PMA for the last 6 h in presence of Brefeldin A. CD3⁺ CD4⁺ T cells were gated and evaluated for IFN-γ production. C) Percentages of CD4⁺IFN-γ⁺ cells in uninfected and 15 d infected WT or IL-17A^−/− mice. Data are representative of 1 of 2 independent experiments with groups of 3–5 mice. *P < 0.05, **P < 0.01.