Figure 7.

Reduced accumulation of neutrophils and Ly6C^hi monocytes in absence of IL-17A. L. donovani–infected WT or IL-17A^−/− mice were euthanized at 15 or 30 dpi. Flow cytometry was performed on cell suspensions from liver and spleen. A) Gating strategy and representative dot plots of immune populations in the liver and spleen of WT or IL-17A^−/− mice at 15 dpi. Neutrophils were identified as CD11b⁺Ly6G^hiLy6C^med (red region), whereas Ly6C^hi monocytes were identified as CD11b⁺Ly6C^hiLy6G⁻ cells (blue region). Gated populations were overlaid on total lymphocytes from liver and spleen and analyzed by Ly6C and CD11b expression. B–E) Kinetic of total number of neutrophils (B, D) and monocytes (C, E) in the liver (B, C) or spleen (D, E) of WT or IL-17A mice during L. donovani infection. F) Aggregates of monocytes and neutrophils were found in WT mice at 15 dpi but not in IL-17A. Liver (15 dpi) sections were examined by confocal microscopy for the expression of Ly6C (magenta), neutrophil elastase (green), and DAPI (blue), DsRed expressing parasites are shown in red (×40 magnification). Data are representative of 2 independent experiments (n = 3–4). *P < 0.05.