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. 2015 Nov 17;30(3):1144–1154. doi: 10.1096/fj.15-277798

Figure 1.

Figure 1.

βAR subtype expression and signaling in MDA-MB-231HM cells. A) mRNA expression of βAR subtypes in MDA-MB-231HM cells (n = 5). B) Saturation binding in MDA-MB-231HM cell membranes using ICYP with/without CGP-20712A (200 nM) or ICI-118551 (50 nM) (n = 4). C) ADRB1 and ADRB2 mRNA was decreased in MDA-MB-231HM cells after expression of targeted siRNA (n = 4). D) Knockdown of β2AR, but not β1AR, abolished 10 nM epinephrine- and 100 nM norepinephrine-induced inhibition of pERK (n = 3). E) βAR agonists caused concentration-dependent increases in cAMP (n = 3–6). F) Only full βAR agonists caused concentration-dependent increases in intracellular Ca2+ (n = 4–5). G) βAR agonists caused concentration-dependent inhibition of basal pERK (n = 3–6). Symbols/bars represent means, error bars sem. ND, not detected. *P < 0.05, **P < 0.01, ***P < 0.001, 1-way ANOVA with Dunnett’s (A–C) or 2-way ANOVA with Tukey’s posttest (D).