Figure 1.

Persistence of vector genome following subretinal injection. A) A standard curve was generated using known DNA concentrations as described in the Materials and Methods. Quantitative PCR was performed using primers for kanamycin resistance gene in the vector at 1 (PI-1m) and 8 mo PI (PI-8m). B) The quantity of DNA was calculated as copy numbers. Data are expressed as mean ± sd (n = 4). Significance was set at the 0.05 level. No statistically significant differences were observed. C) Quantitative PCR products were loaded in 2% agarose gel. β-actin was used a reference gene control.