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Epigenetic regulation of rhodopsin. A) ChIP assay extracted from 6 NP-treated eyes/group at postnatal day 3 were pooled at 1 mo PI and underwent ChIP for H3K4m2, H3K9m1, and H3K27m1 or no Ab followed by quantitative PCR with primers from different regions of the vector. GAPDH intron 2 was used as euchromatin (H3K4m2) control. Mouse AFM intron 2 was used as heterochromatin (H3K9m1 or H3K27m1) control. B) Samples were normalized to the input from the same primer set.
