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. 2015 Nov 12;30(3):1076–1086. doi: 10.1096/fj.15-280511

Figure 5.

Figure 5.

Inflammatory response to the injection. A) Cytokine mRNA quantification by real-time PCR. Retinas were collected and assayed to quantify cytokine gene expression (IL-2, IL-6, TNF-α, and IFN-γ) by RT-PCR at 1 mo PI. Levels of cytokine expression are presented as means ± sd of different samples in triplicate (n = 4). Retinas from untreated and saline-injected mice were used as negative controls. B. cereus-injected eyes were chosen as the positive control. No elevated cytokines were found after the injections in the eyes. B) Immunohistochemistry assessment of infiltrating macrophages after subretinal injection. Immunolabeling with Abs for the macrophage (F4/80, red) and rhodopsin (1D4, green) was performed at 30 d PI in the RKO eyes injected with NP-cRho or -sgRho at P3. Age-matched B. cereus-injected WT eyes were used as positive control. Saline-injected (inj.) and uninjected (uninj.) RKO or WT eyes were used as negative controls. n = 3. ONL, outer nuclear layer; INL, inner nuclear layer. Scale bar, 20 µm.