Fig. 3. : detection and differentiation of Entamoeba histolytica, Entamoeba moshkovskii,Entamoeba dispar and Entamoeba hartmanni by nested-polymerase chain reaction. PCR products were visualised in 1.5% agarose gel with EtBr staining. Line 1: 100 bp DNA ladder; 2, 3: one faecal sample with mixed infection by E. dispar and E. histolytica, respectively; 4, 6, 7: faecal samples positive for E. dispar; 5, 14: empty wells; 8, 9: faecal samples positive for E. histolytica; 10: negative control for E. dispar; 11: negative control for E. histolytica; 12: positive control for E. dispar; 13: positive control for E. histolytica; 15: faecal sample positive for E. hartmanni; 16: negative control.
