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. Author manuscript; available in PMC: 2016 Feb 11.
Published in final edited form as: Methods Mol Biol. 2015;1189:135–162. doi: 10.1007/978-1-4939-1164-6_10

Type of plate Volume of gel/well (μL) Volume of medium/well (μL)

24-Well plate 50–150 750–1,000

4-Well chamber 50–75 750–1,000

2-Well chamber 150–300 1,500–2,000