Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
A schematic illustration of the TMT-based quantitative whole proteomic/phosphoproteomic pipeline. Cells treated with genistein for different time were subjected to FASP-based sample preparation. 6-plex TMT labeling and bRPLC fractionation were carried out and the collected fractions were concatenated. A portion of these fractions were subjected to TiO2 enrichment. Enriched phosphopeptides along with unprocessed peptides were analyzed at an Orbitrap-equipped mass spectrometer.
