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. 2016 Jan 5;48(3):1007–1015. doi: 10.3892/ijo.2016.3323

Figure 1.

Figure 1

IMP3 expression in HSC-1, HSC-5 and HaCaT cells, and normal human skin, and knockdown of IMP3 using siRNA. IMP3 mRNA expression was observed in HSC-1, HSC-5 and HaCaT cell lines and normal skin by RT-qPCR analysis. The expression of IMP3 relative to that in normal skin is shown (A). Knockout of IMP3 mRNA by siRNA in HSC-1, HSC-5 and HaCaT cell lines. IMP3 mRNA is expressed as a percentage of that in siRNA control transfected cells (100%) (B). For (A) and (B), results are expressed as mean ±95% confidence interval. *P<0.0001, compared to normal skin (A) and si-control (siCtrl)-transfected cells (B). Western blot of IMP3 protein expression after treatment with IMP3 siRNA in HSC-1, HSC-5 and HaCaT cell lines. IMP3 protein levels in untransfected wild-type, si control (siCtrl) or siIMP3 RNA-transfected cells are shown (C).