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. 2015 Dec 28;48(3):965–974. doi: 10.3892/ijo.2015.3309

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Copyright: © Tu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Downregulation of miR-519a inhibits the growth of SMMC-7721 cells. (A) The expression of miR-519a was measured in SMMC-7721 cells trans-fected with miR-519a inhibitor or scramble vector. (B) Cell viability was evaluated by the MTT assay at the indicated days. (C) Representative results for colony formation by the indicated cells. (D) Representative micrographs (left) and quantification (right) of BrdU-incorporating cells of indicated SMMC-7721 cells. (E) Effects of miR-519a downregulation on the cell cycle progression of SMMC-7721 cells measured by flow cytometric analysis. Experiments were repeated at least 3 times with similar results, and error bars represent ± SD. ^*P<0.05.