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. 2015 Dec 28;48(3):983–997. doi: 10.3892/ijo.2015.3313

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Copyright: © Katona et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Detection of hyaluronic acid, HAS2 and HAS3 in normal healthy tissues and malignant metastases. Row 1, normal skin, 2, mesenteric lymph node, 3, melanoma metastasis from mesenteric lymph node, 4, normal lung, 5, melanoma metastasis from lung. (A) HA affinity histochemistry. (B) HAS2. (C) HAS3 immunohistochemistry. HA is labelled with Streptavidin-Alexa 555 (red), HAS2 and HAS3 anti-rabbit-Alexa 555 (red). Melanocytes and melanoma cells are shown with MelanA-Alexa 488 (green). Nuclei are demonstrated with DAPI (blue). Scale bar, 50 μm. Representative photomicrographs of 3 independent experiments are shown.