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. 2015 Mar 13;3:28–32. doi: 10.1016/j.ymgmr.2015.03.001

Fig. 1.

Fig. 1

Biochemical and phenotypic analyses. (A) Hepatic microsomal G6Pase-α activity is shown at the indicated ages in weeks (W). Hepatic microsomal G6Pase-α activity in 75–90 week-old wild-type mice (n = 18) averaged 171.8 ± 7.1 nmol/min/mg (100%). The AAV-LL mice (n = 6) expressing 0.5–1.3% of wild-type hepatic G6Pase-α activity are grouped and named based on their hepatic G6Pase-α activity restored relative to wild-type activity as described previously [11]. (B) Blood glucose levels following a 24-hour fast in 38-40 week-old wild-type (n = 8) and AAV-LL (n = 6) mice. (C) Histochemical analysis of hepatic G6Pase-α activity. Freshly sectioned liver specimens were analyzed for G6Pase-α activity using the method of lead trapping of phosphate generated by G6P hydrolysis [14]. Each image represents an individual mouse. (D) Hepatic microsomal G6P uptake activity and G6PT mRNA expression in 75–90 week-old wild-type (n = 8), AAV-LL (n = 6), and 6-week old G6pc−/− (n = 4) mice. (E) Serum metabolite profiles of 75–90 week-old wild-type (n = 18) and AAV-LL (n = 6) mice. (F) Hepatic G6P levels in 75–90 week-old wild-type (n = 23) and AAV-LL (n = 6) mice. (G) Liver and kidney weights of 75–90 week-old wild-type (n = 8) and AAV-LL (n = 6) mice. (−/−), 6-week-old untreated G6pc−/− mice; (+/+), 75–90 week-old wild type mice. Data are mean ± SEM. *P < 0.05, **P < 0.005.