Growth competitions of wild-type HIV-1 (WT; open circles) versus the RT-M184V (A), IN-N155H (B), IN-Q148R (C), RT-M184V + IN-N155H (D), RT-M184V + IN-Q148R (E), RT-K65R/M184V + IN-Q148R (F), and RT-K65R/M184V + IN-N155H (G) mutants (closed circles). Data are averages from 3 independent competition experiments, with standard deviations. (H) Mean relative fitness (RF; 1 + s) values of viruses (genotype indicated) in competition with the WT. Asterisks indicate statistically significant differences for the mutant-versus-WT competitions compared to the WT-versus-WT control competitions using a two-tailed Student's t test (P < 0.01).