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Ratio of catalytic efficiencies (kcat/Km ratios) for wild-type GIM-1 and the GIM-1 mutants toward six β-lactam antibiotics. The kcat/Km ratio for wild-type GIM-1 was set to 1, and the values for the mutants were calculated relative to this. The six β-lactam antibiotics tested were the penicillins ampicillin (AMP) and benzylpenicillin (PEN) (A), the cephalosporins cefoxitin (FOX) and ceftazidime (CAZ) (B), and the carbapenems meropenem (MEM) and imipenem (IPM) (C).
